Expression Information

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Expression levels in RNA sequencing (RNA-Seq) refer to the amount of a specific gene's RNA present in a sample, measured by counting,
how many reads align to that gene. However, raw read counts need to be normalized for factors like gene length and sequencing depth.
RPKM (Reads Per Kilobase of transcript per Million mapped reads) is a common method for this normalization, accounting for gene length
and the total number of reads in the sample. This allows for more accurate comparisons of gene expression across different genes within
the same sample. For cross-sample comparisons, TPM (Transcripts Per Million) or other methods like DESeq2/EdgeR normalization are often
preferred as they address biases that RPKM doesn't fully correct.