Differential Gene Expression (DGE) analysis identifies genes whose expression levels change significantly between different biological conditions. In our study, we aim to compare gene expression in T. myopaeformis prior to and after infection of susceptible and resistant sugar beet genotypes; resistant log2 fold-change in gene expression, we identified T. myopaeformis genes that are upregulated or downregulated in response to how the pest responds to its infection of susceptible and resistant sugar beet genotypes, highlighting the insect’s ability to cope with the sugar beet’s defense mechanisms in resistant varieties and the vulnerability in susceptible ones.
Figure 1. The SBRM resistant sugar beet genotypes F1024 and F1016 and SBRM susceptible sugar beet genotypes F1010 and L19 are analyzed. Naïve SBRM larvae represent the 0 hours post infestation (hpi) time point. SBRM are introduced to the sugar beet genotypes and allowed to infest for 24, 48 or 72 h. The larvae are removed from the sugar beet, flash frozen, having their RNA isolated for RNA sequencing. Comparative analyses between the SBRM resistant sugar beet genotypes F1024 and F1016 to determine similarities and differences in differential gene expression. Analyses are done between the susceptible sugar beet genotypes F1010 and L19 to determine similarities and differences in differential gene expression.
Figure 2. The SBRM resistant sugar beet genotypes F1024 (Resistant genotype 1, Res. 1) and F1016 (Resistant genotype 2, Res. 2) and SBRM susceptible sugar beet genotypes F1010 (Susceptible genotype 1, Susc. 1) and L19 (Susceptible genotype 2, Susc. 2) are analyzed. Naïve SBRM larvae represent the 0 hours post infestation (hpi) time point. SBRM are introduced to the sugar beet genotypes and allowed to infest for 24, 48 or 72 h. The larvae are removed from the sugar beet, flash frozen, having their RNA isolated for RNA sequencing. Comparative analyses between the SBRM resistant sugar beet genotypes F1024 and F1010 (Res. 1 Vs Susc.1) to determine similarities and differences in differential gene expression. Analyses are done between the susceptible sugar beet genotypes F1024 and L19 (Res. 1 Vs Susc.2) to determine similarities and differences in differential gene expression.
Figure 3. The SBRM resistant sugar beet genotypes F1024 (Resistant genotype 1, Res. 1) and F1016 (Resistant genotype 2, Res. 2) and SBRM susceptible sugar beet genotypes F1010 (Susceptible genotype 1, Susc. 1) and L19 (Susceptible genotype 2, Susc. 2) are analyzed. Naïve SBRM larvae represent the 0 hours post infestation (hpi) time point. SBRM are introduced to the sugar beet genotypes and allowed to infest for 24, 48 or 72 h. The larvae are removed from the sugar beet, flash frozen, having their RNA isolated for RNA sequencing. Comparative analyses between the SBRM resistant sugar beet genotypes F1024 and F1010 (Res. 2 Vs Susc.1) to determine similarities and differences in differential gene expression. Analyses are done between the susceptible sugar beet genotypes F1024 and L19 (Res. 2 Vs Susc.2) to determine similarities and differences in differential gene expression.
Figure 4. The SBRM resistant sugar beet genotypes F1024 (Resistant genotype 1, Res. 1) and F1016 (Resistant genotype 2, Res. 2) and SBRM susceptible sugar beet genotypes F1010 (Susceptible genotype 1, Susc. 1) and L19 (Susceptible genotype 2, Susc. 2) are analyzed. Naïve SBRM larvae represent the 0 hours post infestation (hpi) time point. SBRM are introduced to the sugar beet genotypes and allowed to infest for 24, 48 or 72 h. The larvae are removed from the sugar beet, flash frozen, having their RNA isolated for RNA sequencing. Comparative analyses between the common differentially expressed genes found in SBRM resistant sugar beet genotypes F1024 & F1016 (Res. 1 & 2) and the common differentially expressed genes in SBRM susceptible genotypes F1010 & L19 (Susc. 1 & 2) are done to determine similarities and differences in differential gene expression.